Journal of Fisheries and Life Sciences

Author(s): Rohan Avinash Phatak and Sangeeta Sinha

Abstract: The aim of the present investigation is to reveal the antioxidant enzymes response and histopathological alterations in the liver and gill tissues of Catla catla fingerlings to different concentrations of artificial sea water (ASW). LC₅₀ values for the response towards the tolerance for ASW at different time intervals; 24h, 48h, 72h, and 96h and at different concentrations were drawn through the percent mortality determination. The estimation of percent mortality confirmed the fingerlings could tolerate the concentration of ASW up to 47.5 %. Lipid peroxidation assay revealed that the concentration of ASW is directly proportional to the LP levels. The analysis of the antioxidant enzymes (CAT, SOD, GPx & GR) was concentration dependent. CAT & SOD showed an increase while GPx & GR showed a decrease with the increase in concentration of ASW. The reported damage was confirmed by the histopathological analysis that revealed the alterations in gills and liver. The extent of damage to the tissues was proportionate with increased concentration of ASW. The present study clearly demonstrated that Liver and Gill tissues exhibited significant damage with response for artificial sea water toxicity in this species could be possibly used as a model organism for toxicity studies